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Objective@#To explore the causal relationship between self stigma and depression among college students with disabilities, so as to provide reference for mental health promotion among disabled college students.@*Methods@#In October，2021 (T1) and April，2022 (T2)，291 college students from four majors of School of Special Education of Hebei Open University were selected by cluster sampling method to conduct two follow up tests，and online questionnaires were conducted by using Disability Self Stigma Scale，Perceived Social Support Scale and Center for Epidemiological Survey，Depression Scale，and the mediation of perceived social support was established in the cross lag model.@*Results@#The average scores of depressive symptoms of disabled college students in T1 and T2 were (43.51±8.26, 46.82±9.13). The cross lag model showed that T1 self stigma could positively predict T2 depressive( β =0.17, P <0.01). Cross group analysis showed that T1 perceived social support plays a longitudinal mediating role between T1 self stigma and T2 depressive. Predictive effects of self stigma on depressive symptoms in female students ( β =0.42) was stronger than that in males ( β =0.29)( P <0.01). Predictive effects of perceived social support on depressive in female students( β =-0.36) was stronger than that of the males( β =-0.19)( P <0.01).@*Conclusion@#There is a causal relationship between self stigma and depressive symptoms among college students with disabilities. Intervention aim at promoting perceived social support might help to control depressive symptoms.
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ObjectiveTo explore the difference of learning and memory function between permanent cerebral ischemia and ischemia-reperfusion rat models by filament-occluded method after three weeks of natural recovery, and to observe the changes of brain structure. MethodsA total of 21 SPF male Sprague-Dawley rats were randomly divided into sham group (n = 7), permanent ischemia group (n = 7) and ischemia-reperfusion group (n = 7). The latter two group underwent left middle cerebral artery occlusion, and the ischemia-reperfusion group was reperfused after 90 minutes of ischemia. All the rats were tested with Morris Water Maze 16 to 22 days after modeling, to record escape latency in the navigation experiment, as well as first latency, platform quadrant swimming time ratio and distance ratio, boundary swimming time ratio and distance ratio, average speed, and the swimming path in the space exploration experiment. On the 22nd day after modeling, four rats with similar Longa scores in each group were scanned by magnetic resonance by diffusion tensor imaging, to measure the fractional anisotropy (FA) of cerebral ischemic cortex, striatum, hippocampus and their corresponding contralateral regions, to calculate ratio of FA (rFA); as well as the volume of infarct (VI) and ratio of VI (rVI). ResultsFor navigation experiment, the escape latency decreased in all the groups with the time (F = 36.202, P < 0.001), but it was not significant for the permanent ischemia group (F = 4.004, P > 0.05). The escape latency was longer in the permanent ischemia group than in the other two groups at each time points (P < 0.05). For space exploration experiment, the boundary time ratio and distance ratio were more in the permanent ischemia group and the ischemia-reperfusion group than in the sham group (P < 0.017), and the first latency was longer in the permanent ischemia group than in the sham group (P < 0.017). The swimming path of ischemia-reperfusion group and sham operation group was better than that of the permanent ischemia group. FA and rFA in left cortex and striatum were less in the permanent ischemia group and the ischemia-reperfusion group than in the sham group (P < 0.05), while FA and rFA of left cortex and FA of left striatum were less in the permanent ischemia group than in the ischemia-reperfusion group (P < 0.05). The VI and rVI were larger in the permanent ischemia group than in the ischemia-reperfusion group (t > 4.185, P < 0.01). FA and rFA of left cortex and striatum correlated with escape latency, boundary time ratio and distance ratio (|r| > 0.498, P < 0.05). The VI and rVI correlated with escape latency and boundary time ratio (|r| > 0.538, P < 0.05). ConclusionCognitive impairment is more severe in the rat model of permanent cerebral ischemia, which may relate to the larger damage of structure of nerve fibers in ischemic cortex and striatum, and larger infarct size.
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Objective:To investigate the correlation between the door-to-needle time (DNT) delay and the short-term functional outcome after intravenous thrombolysis in patients with minor ischemic stroke and the influencing factors of DNT delay.Methods:From October 2016 to May 2018, patients with minor ischemic stroke received intravenous thrombolysis with alteplase from the Stroke Database of Nanjing First Hospital were enrolled retrospectively. DNT delay was defined as DNT > median. The modified Rankin Scale was used to evaluate the short-term functional outcome at 3 months after stroke. 0-1 was defined as good outcome, and ≥2 was defined as poor outcome. Univariate analysis was used to evaluate the correlation between DNT delay and short-term functional outcome. Multivariate logistic regression analysis was used to evaluate the possible influencing factors of DNT delay. Results:A total of 102 patients with minor ischemic stroke were enrolled. The median DNT was 40 min, 36 patients (35.3%) had DNT delay, and 27 patients (26.5%) had poor short-term outcome. Univariate analysis showed that there was no significant difference in the proportion of patients with DNT delay between the poor outcome group and the good outcome group (44.4% vs. 32.0%; χ2=1.346, P=0.252). Multivariate logistic regression analysis showed that there was a significant independent negative correlation between hypertension and DNT delay (odds ratio 0.359, 95% confidence interval 0.137-0.939; P=0.037). Conclusion:For patients with minor ischemic stroke receiving intravenous thrombolysis, DNT delay is not associated with the outcome. The absence of hypertension may be one of the factors affecting the DNT delay.
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Objective:To investigate the correlation between the high-resolution magnetic resonance imaging (MRI) vessel wall characteristics and risk of ischemic stroke in patients with middle cerebral artery (MCA) atherosclerotic stenosis.Methods:Patients with ischemic cerebrovascular disease treated in the Department of Neurology, Nanjing First Hospital, Nanjing Medical University from January 2016 to January 2020 were enrolled prospectively. The patients presented with transient ischemic attack or acute ischemic stroke (AIS). Magnetic resonance angiography showed that the ipsilateral MCA had stenosis of 50%-99%, and it was identified as the responsible lesion. Routine MRI and the vessel wall imaging at the narrowest part of MCA were performed. The characteristics of vessels and plaques at MCA stenosis were compared between the AIS group and the non-AIS group. Multivariate logistic regression analysis was used to determine the independent risk factors for AIS. Receiver operating characteristic (ROC) curve was used to determine the predictive value of vessel wall characteristics for AIS. Results:A total of 65 patients with ischemic cerebrovascular disease and MCA stenosis were enrolled. The age of patients was 68±14 years, 50 were males (76.9%). There were 30 patients (46.2%) in the AIS group and 35 (53.8%) in the non-AIS group. There were no significant differences in demographic data, vascular risk factors and routine laboratory tests between the two groups. Compared with the non-AIS group, the narrowest lumen area in the AIS group (2.36±1.09 mm 2vs. 2.96±1.01 mm 2; t=2.274, P=0.027) was smaller, the plaque area (4.46 ±2.08 mm 2vs. 2.62±1.32 mm 2; t=4.315, P<0.001) was larger, the remodeling index (1.08±0.11 vs. 0.94±0.10; t=5.573, P<0.001) was higher, and the proportion of obvious enhanced plaque (63.3% vs. 11.4%; χ2=19.034, P<0.001) and positive remodeling plaque (80.0% vs. 20.0%; χ2=23.311, P<0.001) were higher. Multivariate logistic regression analysis showed that plaque area (odds ratio [ OR] 2.01, 95% confidence interval[ CI] 1.35-2.98; P=0.001), remodeling pattern ( OR 16.00, 95% CI 4.73-54.15; P=0.001), the narrowest lumen area ( OR 0.568, 95% CI 0.34-0.96; P=0.033) and degree of enhancement ( OR 21.85, 95% CI 5.13-93.00; P<0.001) were the independent risk factors for AIS. ROC curve analysis showed that the combination of plaque area, the narrowest lumen area and the degree of enhancement had the best prediction effect (area under the curve 0.927, 95% CI 0.84-0.96). Conclusion:AIS is more likely to occur when the plaque area at the narrowest part of the MCA is larger, the lumen area is smaller, and there is obvious plaque enhancement.
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Objective:To explore the clinical application and curative effect of brachytherapy of denture applicators with 125I seeds in the treatment of palatal malignant tumor. Methods:Thirty patients with palatal malignant tumor who underwent surgical resection in Shandong Provincial Hospital from February 2012 to January 2020, and brachytherapy was performed with applicator additional 125I seeds 2 weeks after surgery. All patients were followed up for treatment effect and adverse reactions. Results:All patients wore 125I seeds denture base denture applicator well, no 125I seeds displacement and loss. 30 patients had 10-60 months of follow-up, among which 1 patient received palliative treatment with 125I seeds denture applicator alone died after 10 months of follow-up; 1 patient with adenoid cystic carcinoma recurred after 2 years of follow-up and underwent surgical treatment again; the rest patients did not see tumor recurrence or metastasis. The side effects, pronunciation and chewing function were improved in patients ( P<0.05). Conclusions:For patients with palatal malignant tumor, postoperative 125I seeds denture applicator can effectively prevent tumor recurrence and metastasis, reduce complications, and improve the quality of life.
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@#Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths, characterized by highly hypoxic tumor microenvironment. Hypoxia-inducible factor-1α (HIF-1α) is a major regulator involved in cellular response to changes of oxygen levels, supporting the adaptation of tumor cells to hypoxia. Bruceine D (BD) is an isolated natural quassinoid with multiple anti-cancer effects. Here, we identified BD could significantly inhibit the HIF-1α expression and its subsequently mediated HCC cell metabolism. Using biophysical proteomics approaches, we identified inhibitor of β-catenin and T-cell factor (ICAT) as the functional target of BD. By targeting ICAT, BD disrupted the interaction of β-catenin and ICAT, and promoted β-catenin degradation, which in turn induced the decrease of HIF-1α expression. Furthermore, BD could inhibit HCC cells proliferation and tumor growth in vivo, and knockdown of ICAT substantially increased resistance to BD treatment in vitro. Our data highlight the potential of BD as a modulator of β-catenin/HIF-1α axis mediated HCC metabolism.
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Cashmere, also known as soft gold, is produced from the secondary hair follicles (SHFs) of cashmere goats. The number of SHFs determines the yield and quality of cashmere; therefore, it is of interest to investigate the transcriptional profiles present during cashmere goat hair follicle development. However, mechanisms underlying this development process remain largely unexplored, and studies regarding hair follicle development mostly use a murine research model. In this study, to provide a comprehensive understanding of cellular heterogeneity and cell fate decisions, single-cell RNA sequencing was performed on 19,705 single cells of the dorsal skin from cashmere goat fetuses at induction (embryonic day 60; E60), organogenesis (E90), and cytodifferentiation (E120) stages. For the first time, unsupervised clustering analysis identified 16 cell clusters, and their corresponding cell types were also characterized. Based on lineage inference, a detailed molecular landscape was revealed along the dermal and epidermal cell lineage developmental pathways. Notably, our current data also confirmed the heterogeneity of dermal papillae from different hair follicle types, which was further validated by immunofluorescence analysis. The current study identifies different biomarkers during cashmere goat hair follicle development and has implications for cashmere goat breeding in the future.
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Liver injury caused by acetaminophen (AP) overdose is a leading public health problem. Although AP-induced liver injury is well recognized as the formation of N-acetyl-p-benzoquinone (NAPQI), a toxic metabolite of AP, resulting in cell damage, emerging evidence indicates that AP-induced liver injury is also associated with gut microbiota. However, the gut microbiota-involved mechanism remains largely unknown. In our study, we found that vancomycin (Vac) pretreatment (100 mg/kg, twice a day for 4 days) attenuated AP-induced liver injury, altered the composition of gut microbiota, and changed serum metabolic profile. Moreover, we identified Vac pretreatment elevated cecum and serum 2-hydroxybutyric acid (2-HB), which ameliorated AP-induced cell damage and liver injury in mice by reducing AP bioavailability and elevating GSH levels. Our current results revealed the novel role of 2-HB in protecting AP-induced liver injury and add new evidence for gut microbiota in affecting AP toxicity.
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Obejctive To determine and compare britanin content in dried aerial parts and capitulum of Inula linariifolia Turcz from 7 different origins by HPLC. Methods Analysis was performed on Agilent Zorbax SB C18 (250 mm×4.6 mm, 5 μm). Acetonitrile and water were used as mobile phase for gradient elution at 1.2 ml/min. Column temperature was 30 ℃ and the detection wavelength at 212 nm. Results The results meet the requirements of the method validation in 2015 edition of Chinese Pharmacopeia. The average britanin content in dried aerial parts of Inula linariifolia Turcz is 0.125% vs 0.732% in capitulum, which is significantly different. Conclusion The established method is simple and convenient. It can be used for quality control of Inula linariifolia Turcz.
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Objective To investigate the effects of calycosin, formononetin, calycosin-7-glucoside and ononin on PC 12 cells differentiation. Methods PC 12 cells were cultured and treated with different concentrations of nerve growth factor (NGF), calycosin, formononetin, calycosin-7-glucoside and ononin for 5 days, once a day, 3 times in a row. The neurite outgrowth of PC 12 cells was observed and the expression of β III-tubulin were measured by immunofluorescence. Results Compared with the vehicle group, neurite outgrowth and the expression of β III tubulin in PC 12 cells had not promoted by calycosin, formononetin, calycosin-7-glucoside and ononin (0.01-10.00 μmol/L). Conclusion PC 12 cells differentiation could not be induced by calycosin, formononetin, calycosin-7-glucoside and ononin.
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Since December 2019, Corona Virus Disease (COVID-19), a new emerging infection disease occurred in Wuhan, has spread in 27 countries and regions. The clusters of many cases were reported with the epidemic progresses. We collected currently available information for 377 COVID-19 clusters (1 719 cases), excluded the hospital clusters and Hubei cases, during the period from January 1, 2020 to February 20, 2020. There were 297 family clusters (79%), case median 4; 39 clusters of dining (10%), case median 5; 23 clusters of shopping malls or supermarkets (6%), case median 13; 12 clusters of work units (3%), case median 6, and 6 clusters of transportation. We selected 325 cases to estimate the incubation period and found its range is 1 to 20 days, median was 7 days, and mode was 4 days. The analysis of the epidemic situation in a department store in China indicates that there is a possibility of patients as the source of infection during the incubation period of the epidemic. From February 5, 2020 to February 21, 2020, 634 persons were infected in the Diamond Princess Liner. All persons are susceptible to SARS-CoV-2. The older, patients during the incubation period and the worse environment may be the cause of the cases rising. The progress of the two typical outbreaks clearly demonstrates the spread of the early cases in Wuhan. Whatever happens, screening and isolating close contacts remains essential except for clinical treatment during the epidemic. Especially for the healthy people in the epidemic area, isolation is the key.
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Objective@#To explore the effect of expanded flaps with vascular anastomosis in the treatment of scar contracture deformities of extensively burned patients.@*Methods@#From April 2016 to February 2018, 9 patients with severe scar contracture deformities caused by extensive burns were hospitalized in our unit, including 7 males and 2 females, aged 23-54 years. There were 14 sites of scar contracture deformities and dysfunction, including 8 in face and neck, 2 in elbow, and 4 in wrist and hand. The expander was inserted into the chest or abdomen and was expanded by 2 to 3 times of its rated volume with injection of normal saline. After satisfied expansion, the expanded flap was harvested and transplanted with arteriovenous anastomosis onto the recipient site, where the scar was removed, the deformity was corrected, and the contracture was released. The number of expanders, the volume of normal saline injected, the period of expansion, the complications of skin and soft tissue expansion, the number, size, thickness, transplantation modes, and survival of flaps, and the repair method of donor site were observed and recorded. The reconstruction effect of scar contracture deformity was followed up. The patients′ satisfaction with the therapeutic effect of various surgical sites during follow-up was investigated with a 5-point Likert Scale. The Burn Specific Health Scale-Brief was used to evaluate the quality of life of the patients pretreatment and during follow-up. Data were processed with paired sample t test or Wilcoxon signed-rank sum test.@*Results@#A total of 16 expanders were inserted in this group of patients, including 6 in the chest and 10 in the abdomen. The volume of normal saline injected at the end of expansion was (1 421±348) mL. The expansion time was (8.1±2.6) months. One case of expander leakage and one case of injection port turnover resulted in failure of water injection occurred during expansion. Totally 17 flaps were resected from 16 expanded areas. The size of flaps ranged from 15 cm×13 cm to 30 cm×25 cm. The thickness was (0.49±0.06) cm in 6 chest flaps and (0.76±0.15) cm in 11 abdomen flaps. Free transplantation with vascular anastomosis was performed in 14 flaps, and pedicled transplantation supercharged with distal vascular anastomosis was performed in 3 flaps, one of which the vascular pedicle was divided and re-anastomosed to the other side of the recipient area 2 weeks later. Except for one donor site of abdomen flap which was transplanted with thin split-thickness scalp, the other donor sites were sutured directly. After operation, 2 flaps were slightly necrotic at the distal end and healed after dressing change and thin split-thickness scalp transplantation respectively, while the remaining 15 flaps all survived. During the follow-up of 6-24 months, the texture of the flaps was soft. The abdomen flap transplanted to the palm of hand in one patient was slightly hypertrophic, which was thinned 3 months after operation, while the other flaps were good in thickness. At the last follow-up, the appearance and function of the sites repaired by flaps of all patients were obviously improved compared with those before operation, the satisfaction score of the patients with the therapeutic effect of the surgical site was (4.4±0.6) points, the total score of quality of life and the scores of heat sensitivity, treatment antipathy, body image, and affect of patients were significantly higher than those before treatment (t=3.232, 2.683, 3.969, 2.884, 2.588, P<0.05), while the scores of hand function, sexuality, interpersonal relationship, simple function abilities, perception in returning to work of patients were close to those before treatment (t=0.778, 1.000, 1.664, Z=1.826, 1.633, P>0.05).@*Conclusions@#Expanded flaps with vascular anastomosis are suitable for the treatment of scar contracture deformities of extensively burned patients. The flaps are large in size and suitable in thickness. The donor sites are easy to be closed directly. The treatment can obviously improve the appearance, function, and the quality of life of the patients, with a high satisfaction of patients.
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Objective To analyze the influence from size parameters of minimally invasive vascular clamp on mechanical properties of small arteries. Methods The finite element simulation analysis on the process of minimally invasive vascular clamp clamping small arteries was performed. The influence patterns of 5 different sawtooth spacing, sawtooth heights and sawtooth lengths on mechanical properties of small arteries were studied. Results Larger sawtooth spacing led to smaller maximum equivalent stress of the clamped artery. The maximum equivalent stress of the small artery was not linear with the sawtooth height of the vascular clamp. The maximum equivalent stress of the small artery was the smallest and the vascular injury was the minimal when the swatooth height was 75 μm. The sawtooth length of the vascular clamp had an important influence on mechanical properties of clamped small arteries. The maximum equivalent stress of the artery was proportional to the sawtooth length of the vascular clamp. Conclusions The size parameters of minimally invasive vascular clamp had an important influence on mechanical properties in the process of clamping small arteries. The research findings can provide guidance for the design of the minimally invasive vascular clamp.
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Objective@#To explore the clinical efficacy of lobulated transplantation of free anterolateral thigh perforator flap in repairing electric burn wounds of limbs.@*Methods@#From August 2014 to April 2019, 19 patients with electric burns in the limbs were hospitalized in our unit, including 18 males and 1 female, aged 20-58 years. There were 37 wounds deep to bone. The area of wounds ranged from 3.0 cm×2.0 cm to 40.0 cm×8.0 cm. Multiple-perforator-based anterolateral thigh flap was designed and resected. Then the flap was lobulated taking the respective perforators of the lateral circumflex femoral artery as the axial vessels before being transplanted to the debrided wounds in the limbs. The blood vessel trunk or the perforator vessels of flap lobes were anastomosed with the respective vessels in the recipient sites. The wounds were repaired with respective lobes of the flap when repairing multiple wounds in one surgical procedure, whereas the lobes were spliced or staggered to cover the wound to fit the shape of wound when repairing a single irregular wound in one surgical procedure. For the limb with distal blood supply disorder, the blood supply branch of flap was used to reconstruct the blood supply. If necessary, an appropriate length of vein was taken for transplantation. The improvement of reconstructed blood supply was observed. The number of surgeries, the number of anterolateral thigh perforator flaps, the number and size of flap lobes, the number of anastomosed vessels in each surgery, the treatment of the donor sites, the length of each surgery, the postoperative complications and survival condition of flap lobes were recorded. The upper extremity function was evaluated with the Carroll′s Upper Extremity Function Test Scale, and the patients′ satisfaction degree with the therapeutic effect of each surgery was investigated with a 5-point Likert Scale during follow-up. Surgeries were divided into single wound group of repairing one wound at one time and multiple wounds group of repairing two or more wounds at one time. The number of anastomosed vessels in each surgery, the treatment of the donor sites, the length of each surgery, and the postoperative survival condition of the flap lobes were compared between the two groups. Surgeries were divided into early group of performing surgery within post burn day 7 and late group of performing surgery on post burn day 7 and beyond. The postoperative complications and survival condition of flap lobes, the evaluation score of upper limb function and the patients′ satisfaction degree with the therapeutic effect of each surgery at the last follow-up were compared between the two groups. Data were processed with independent sample t test, Mann-Whitney U test, or Fisher′s exact probability test.@*Results@#The blood supply of 5 patients with distal hand or finger blood supply disorder recovered or improved significantly after vascular transplantation. A total of 46 lobes [(2.2±0.4) lobes per flap] were obtained from 21 anterolateral thigh perforator flaps in 19 patients with 21 surgeries. The area of flap lobes ranged from 4.0 cm×3.0 cm to 24.0 cm×13.0 cm. In each surgery, 2.0 (1.5, 3.0) arteries and 3.0 (2.0, 3.0) veins were anastomosed. Six donor sites were repaired by thin split-thickness scalp, and 15 donor sites were closed directly. The duration of each surgery was (8.9±1.7) h. After surgery, bleeding and hematoma occurred in 2 flap lobes and local infection occurred in 5 flap lobes, which were improved after management. Vascular crisis occurred in 4 flap lobes, and exploratory surgeries were performed, after which 2 lobes survived, while the other 2 lobes necrotized and were repaired by other methods. The rest flap lobes survived well. After each postoperative follow-up of 3 to 60 months, the flap covering areas of the limbs were well-recovered. At the last follow-up, the function evaluation score of 20 affected upper limbs was 85 (63, 90) points, and the score of patients′ satisfaction degree with the therapeutic effect of each surgery was (4.4±0.7) points. A total of 30 flap lobes were obtained in 14 surgeries and repaired 30 wounds respectively in multiple wounds group, and 16 flap lobes were obtained in 7 surgeries and were spliced to repair 7 large irregular wounds in single wound group. There were no statistically significant differences in the number of anastomosed artery or vein in each surgery, and the duration of each surgery between multiple wounds group and single wound group (Z=0.240, 0.081, t=0.180, P>0.05), and the condition of skin grafting in the donor sites and the postoperative survival of the flap lobes in multiple wounds group were similar to those in single wound group (P>0.05). A total of 22 flap lobes were obtained in 10 surgeries and repaired 18 wounds in early group, and 24 flap lobes were obtained in 11 surgeries and repaired 19 wounds in late group. The incidence of postoperative hematoma, infection, vascular crisis, and survival of flap lobes in early group were similar to those in late group (P>0.05). There were no statistically significant differences in the patients′ satisfaction degree with the therapeutic effect of each surgery at the last follow-up between early group and late group (t=0.701, P>0.05). At the last follow-up, the function evaluation score of 9 upper limbs in early group was 90 (85, 97) points, significantly higher than 80 (40, 85) points of 11 upper limbs in late group (Z=2.431, P<0.05).@*Conclusions@#Free lobulated anterolateral thigh perforator flap is suitable for simultaneous repair of multiple electric burn wounds of limbs, as well as the repair of a single large irregular wound. It has the clinical advantages of less damage to the donor site and good repair quality. The early flap transplantation is beneficial to improve the function of limbs with electric burns.
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Objective@#To investigate the spatial relationship between intraparotid facial nerve and parotid ducts in different types of parotid neoplasms with 3D improved motion-sensitized driven-equilibrium pulse (3D-iMSDE) MR neurography, and to guide the operation plan.@*Methods@#By means of prospective research methods, the sequences of 3D-iMSDE and 3D-T2-fast field echo (3D-T2-FFE) were obtained in 30 healthy volunteers and 40 patients with parotid gland tumors, respectively. All patients with parotid mass were found by physical examination, by maxillofacial surgeons or by ultrasonography or other imaging examinations. Facial nerve trunk with its first branches and parotid ducts were evaluated independently on images of iMSDE and T2-FFE sequence for volunteers. The signal intensity ratio (SIR) of both facial nerve and parotid ducts were compared respectively in two sequences The relationship between intraparotid facial nerve and parotid ducts with the lesion were analyzed on images of iMSDE and T2-FFE sequences for 40 patients. The results were compared with intra-operative anatomy and post-operative pathological findings.Wilcoxon symbolic rank test, t test and chi-square test were used@*Results@#The image scores of intra-parotid facial nerve and parotid ducts in iMSDE sequence were higher than that of T2-FFE sequence in all 30 healthy volunteers. The difference was statistically significant (Z=-6.197,P<0.05). Both the facial nerve and parotid duct on images of iMSDE sequence had higher SIR than that of T2-FFE. The differences were statistically significant (t=10.772,11.586, respectively;P<0.05). Forty patients with parotid gland tumors had a total of 41 lesions. Compared with the intraoperative anatomy, the accuracy of iMSDE and T2-FFE sequences in showing the relationship between the facial nerve and its primary branches with the mass were 36/41 (87.8%), and 22/41 (53.7%) respectively, and the accuracy of the two sequences in showing the relationship between the dominant duct of the parotid gland and the mass were 38/41 (92.7%) and 30/41 (73.2%), respectively.The accuracy rate in showing the relationship between facial nerve and its primary branch and the mass on images of iMSDE sequence were higher than that of T2-FFE sequence, and also in showing the relationship of parotid duct and the mass. The statistical differences were significant (χ2=9.725, 5.513;P<0.05,respectively). In the iMSDE sequence, the relationship between the facial nerve and its first branches (temporofacial and cervicofacial division) with the tumor was correctly demonstrated in 36 cases. 38 cases of parotid ducts were satisfactorily displayed on image of iMSDE sequence.@*Conclusions@#The 3D-iMSDE MRN sequence can correctly show the relationship between intraparotid facial nerve and parotid duct and the parotid gland neoplasms.
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Objective@#To explore the diagnostic value of serum glutamyltransferase (GGT) and osteopontin (OPN) for asymptomatic secondary common bile duct stones.@*Methods@#A total of 150 gallbladder stones patients were divided into asymptomatic secondary common bile duct stones patients (common bile duct stones group) 34 cases and gallbladder stone group 116 cases. The difference of serum liver function indexes and OPN concentration between 2 groups were compared. Meanwhile, the diagnostic value of serum GGT combined with OPN was assessed by receiver operating characteristic curve.@*Results@#There were no statistical difference in alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBil) and direct bilirubin (DBil) between 2 groups (P>0.05). The GGT, alkaline phosphatase (ALP) and OPN in common bile duct stones were significantly higher than those in gallbladder stone group: (141.57 ± 47.11) U/L vs. (77.26 ± 21.76) U/L, (150.10 ± 17.55) U/L vs. (137.65 ± 24.36) U/L and (16.21 ± 3.26) mg/L vs. (11.50 ± 3.99) mg/L, there were statistical differences (P<0.05). Logistic multivariate analysis showed that GGT, ALP and OPN were the independent risk factors of asymptomatic secondary common bile duct stones (P<0.05). The areas under curve of GGT and OPN in diagnosis of asymptomatic secondary common bile duct stones were significantly higher than ALP (P<0.05).@*Conclusions@#Serum GGT and OPN can be served as early predictors for the gallbladder stone combine with asymptomatic secondary common bile duct stones.
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Objective To explore the diagnostic value of serum glutamyltransferase (GGT) and osteopontin (OPN) for asymptomatic secondary common bile duct stones. Methods A total of 150 gallbladder stones patients were divided into asymptomatic secondary common bile duct stones patients (common bile duct stones group) 34 cases and gallbladder stone group 116 cases. The difference of serum liver function indexes and OPN concentration between 2 groups were compared. Meanwhile, the diagnostic value of serum GGT combined with OPN was assessed by receiver operating characteristic curve. Results There were no statistical difference in alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBil) and direct bilirubin (DBil) between 2 groups (P＞0.05). The GGT, alkaline phosphatase (ALP) and OPN in common bile duct stones were significantly higher than those in gallbladder stone group: (141.57 ± 47.11) U/L vs. (77.26 ± 21.76) U/L, (150.10 ± 17.55) U/L vs. (137.65 ± 24.36) U/L and (16.21 ± 3.26) mg/L vs. (11.50 ± 3.99) mg/L, there were statistical differences (P﹤0.05). Logistic multivariate analysis showed that GGT, ALP and OPN were the independent risk factors of asymptomatic secondary common bile duct stones (P﹤0.05). The areas under curve of GGT and OPN in diagnosis of asymptomatic secondary common bile duct stones were significantly higher than ALP (P﹤0.05). Conclusions Serum GGT and OPN can be served as early predictors for the gallbladder stone combine with asymptomatic secondary common bile duct stones.
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Objective To investigate the spatial relationship between intraparotid facial nerve and parotid ducts in different types of parotid neoplasms with 3D improved motion-sensitized driven-equilibrium pulse (3D-iMSDE) MR neurography, and to guide the operation plan. Methods By means of prospective research methods, the sequences of 3D-iMSDE and 3D-T2-fast field echo (3D-T2-FFE) were obtained in 30 healthy volunteers and 40 patients with parotid gland tumors, respectively. All patients with parotid mass were found by physical examination, by maxillofacial surgeons or by ultrasonography or other imaging examinations. Facial nerve trunk with its first branches and parotid ducts were evaluated independently on images of iMSDE and T2-FFE sequence for volunteers. The signal intensity ratio (SIR) of both facial nerve and parotid ducts were compared respectively in two sequences The relationship between intraparotid facial nerve and parotid ducts with the lesion were analyzed on images of iMSDE and T2-FFE sequences for 40 patients. The results were compared with intra-operative anatomy and post-operative pathological findings.Wilcoxon symbolic rank test, t test and chi-square test were used Results The image scores of intra-parotid facial nerve and parotid ducts in iMSDE sequence were higher than that of T2-FFE sequence in all 30 healthy volunteers. The difference was statistically significant (Z=-6.197,P<0.05). Both the facial nerve and parotid duct on images of iMSDE sequence had higher SIR than that of T2-FFE. The differences were statistically significant (t=10.772,11.586, respectively;P<0.05). Forty patients with parotid gland tumors had a total of 41 lesions. Compared with the intraoperative anatomy, the accuracy of iMSDE and T2-FFE sequences in showing the relationship between the facial nerve and its primary branches with the mass were 36/41 (87.8%), and 22/41 (53.7%) respectively, and the accuracy of the two sequences in showing the relationship between the dominant duct of the parotid gland and the mass were 38/41 (92.7%) and 30/41 (73.2%), respectively.The accuracy rate in showing the relationship between facial nerve and its primary branch and the mass on images of iMSDE sequence were higher than that of T2-FFE sequence, and also in showing the relationship of parotid duct and the mass. The statistical differences were significant (χ2=9.725, 5.513;P<0.05,respectively). In the iMSDE sequence, the relationship between the facial nerve and its first branches (temporofacial and cervicofacial division) with the tumor was correctly demonstrated in 36 cases. 38 cases of parotid ducts were satisfactorily displayed on image of iMSDE sequence. Conclusions The 3D-iMSDE MRN sequence can correctly show the relationship between intraparotid facial nerve and parotid duct and the parotid gland neoplasms.
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Methods: Twenty Sprague Dawley rats were randomly divided into 2 groups ( n=10). DermalGen were implanted subcutaneously into the dorsum of rats in experimental group, and the rats in control group were treated with sham-operation. At 3, 7, and 15 days and 1, 3, 6, and 12 months after operation, the samples of experimental group were harvested and gross observation, histological observation, CD31 immunohistochemical staining, and transmission electron microscope observation were taken to observe the inflammatory reaction, angiogenesis, and collagen arrangement. The skin tissues of the control group at 12 months were observed and compared.
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Objective To explore the association of serum and sputum desmosine with treatment response in patients with chronic obstructive pulmonary disease(COPD). Methods Serum and induced sputum desmosine were measured with enzyme linked immunosorbent assay in 65 patients with newly diagnosed COPD and 26 healthy people. The associations of desmosine with pulmonary function ,modified Medical Research Council dyspnea scale(mMRC),and St. George's Respiratory Questionnaire score(SGRQ)were analyzed before and after treat-ment with inhaled corticosteroid/long acting β2-agonist. The relationship between desmosine and treatment re-sponse in COPD were explored. Results Level of sputum desmosine was higher in patients with COPD than in healthy controls(1061.2 ± 933.9 ng/mL vs. 443.5 ± 501.7 ng/mL;t=2.277,P=0.027). Sputum desmosine level was negatively related with forced expiratory volume in 1 second(FEV1)(r=-0.357,P=0.001)and forced vital capacity(FVC)(r =-0.479,P = 0.02). Serum desmosine level was correlated with pulmonary function,MRC, and SGRQ(P>0.05 for all comparisons). 3 months after treatment,neither serum nor sputum desmosine declined significantly(P>0.05). FVC,MRC,and the total scores and activity scores on the SGRQ improved more markedly in patients with lower expression of sputum desmosine than in those with higher expression(P < 0.05 for all com-parisons). Conclusions Level of sputum desmosine is inversely correlated with pulmonary function in stable COPD. Patients with lower expression of sputum desmosine have more significant improvement in symptoms.